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dls  (Malvern Panalytical)


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    Malvern Panalytical dls
    Dls, supplied by Malvern Panalytical, used in various techniques. Bioz Stars score: 99/100, based on 214545 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dls/Zetasizer+Advance/10__34294_slash_j__jsta__26__21__122-73-9-14
    Average 99 stars, based on 214545 article reviews
    dls - by Bioz Stars, 2026-09
    99/100 stars

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    ( A ) Schematic representation of the experimental design to quantify the arginine methylproteome in IVDD. ( B ) Upset plot indicating the presence of arginine methylated peptides identified in each sample. ( C ) Liquid chromatography–tandem mass spectrometry of the tryptic peptide FDPVGPLPGPNPILPGRGGPNDR identified a monomethylated residue at R504. ( D ) Methyltransferase inhibitors decrease FBXO7 methylation. GFP-tagged FBXO7 was expressed in HEK293cells, followed by treatment with increasing concentrations of the methyltransferase inhibitor AdOx. ( E ) Co-IP analysis of methylated FBXO7 in NPCs with the indicated treatment. ( F ) Co-IP analysis of FBXO7-R504me in NPCs with the indicated treatment. ( G ) Co-IP analysis of FBXO7-R504me in P2 and P8 NPCs. ( H ) IHC of the FBXO7-R504me in human NP tissue. Scale bar: 100 μm. ( I ) IF staining of FBXO7-R504me in coccygeal vertebrae from rats with the indicated treatment. Scale bars: 1 mm (left), 250 μm (right). At least 3 independent experiments were performed.

    Journal: The Journal of Clinical Investigation

    Article Title: Dysregulated methylation-ubiquitination crosstalk accelerates intervertebral disc degeneration via MED12 destabilization and cGAS/STING activation

    doi: 10.1172/JCI197274

    Figure Lengend Snippet: ( A ) Schematic representation of the experimental design to quantify the arginine methylproteome in IVDD. ( B ) Upset plot indicating the presence of arginine methylated peptides identified in each sample. ( C ) Liquid chromatography–tandem mass spectrometry of the tryptic peptide FDPVGPLPGPNPILPGRGGPNDR identified a monomethylated residue at R504. ( D ) Methyltransferase inhibitors decrease FBXO7 methylation. GFP-tagged FBXO7 was expressed in HEK293cells, followed by treatment with increasing concentrations of the methyltransferase inhibitor AdOx. ( E ) Co-IP analysis of methylated FBXO7 in NPCs with the indicated treatment. ( F ) Co-IP analysis of FBXO7-R504me in NPCs with the indicated treatment. ( G ) Co-IP analysis of FBXO7-R504me in P2 and P8 NPCs. ( H ) IHC of the FBXO7-R504me in human NP tissue. Scale bar: 100 μm. ( I ) IF staining of FBXO7-R504me in coccygeal vertebrae from rats with the indicated treatment. Scale bars: 1 mm (left), 250 μm (right). At least 3 independent experiments were performed.

    Article Snippet: To explore the effects of arginine methylation, 20 or 50 μM AdOx (MedChemExpress), a PRMT inhibitor, was used to treat NPCs or HEK293T cells.

    Techniques: Methylation, Liquid Chromatography, Mass Spectrometry, Residue, Co-Immunoprecipitation Assay, Staining

    Schematic illustration showing a mechanism linking methylation-ubiquitination crosstalk of MED12 and R-loop/cGAS/STING axis in NPC senescence and IVDD progression.

    Journal: The Journal of Clinical Investigation

    Article Title: Dysregulated methylation-ubiquitination crosstalk accelerates intervertebral disc degeneration via MED12 destabilization and cGAS/STING activation

    doi: 10.1172/JCI197274

    Figure Lengend Snippet: Schematic illustration showing a mechanism linking methylation-ubiquitination crosstalk of MED12 and R-loop/cGAS/STING axis in NPC senescence and IVDD progression.

    Article Snippet: To explore the effects of arginine methylation, 20 or 50 μM AdOx (MedChemExpress), a PRMT inhibitor, was used to treat NPCs or HEK293T cells.

    Techniques: Methylation, Ubiquitin Proteomics